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Item Number：C3138
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Specification & Item NumberC3138-0500(500mL)
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Storage Conditions2-8℃，keep out of the light
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Shipping Conditionroom temperature
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Shelf Life(Months)12 months
Product Introduction
Ham's F-12K (Kaighn's) medium is an improved version of Ham's F-12 nutrient mixture.
The culture medium has been supplemented with L-Alanyl-L-Glutamine in place of L-Glutamine. Compared with L-Glutamine, L-Alanyl-L-Glutamine is much more stable, releases less ammonia in culture, and improves cell viability and growth.
This medium does not contain proteins or growth factors, so it is usually necessary to supplement it with fetal bovine serum (FBS) and growth factors. The concentration of FBS required varies among different cell lines, and it is recommended to optimize it for specific cell types. Additionally, Ham's F-12K uses a sodium bicarbonate buffer system (2.5 g/L), and it should be used in an environment with 5–10% CO₂ to maintain physiological pH.
Product Application
Ham's F12K (Kaighn's) medium is designed for primary human hepatocytes and rat and chicken hepatocytes that can grow under low serum conditions. Compared with traditional basal media, Ham's F-12K contains additional putrescine, thymidine, hypoxanthine, zinc, and higher concentrations of all amino acids and sodium pyruvate. These components enable certain cell types to support growth with only a minimal amount of serum or defined chemical additives.
Production And Quality System
Manufactured in accordance with cGMP guidelines and in line with ISO13485 manufacturing standards.
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